Tumor Cell Detection by Mass Spectrometry Using Signal Ion Emission Reactive Release Amplification.
Anal Chem
; 88(14): 6971-5, 2016 07 19.
Article
em En
| MEDLINE
| ID: mdl-27351295
ABSTRACT
A method is presented for the detection of circulating tumor cells (CTC) using mass spectrometry (MS), through reporter-ion amplification. Particles functionalized with short-chain peptides are bound to cells through antibody-antigen interactions. Selective release and MS detection of peptides is shown to detect as few as 690 cells isolated from a 10 mL blood sample. Here we present proof-of-concept results that pave the way for further investigations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Células Neoplásicas Circulantes
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos