Decreased expression of Beclin1 is significantly associated with a poor prognosis in oral tongue squamous cell carcinoma.
Mol Med Rep
; 14(2): 1567-73, 2016 Aug.
Article
em En
| MEDLINE
| ID: mdl-27356955
ABSTRACT
The autophagy-related gene Beclin-1 is critical in the regulation of tumourigenesis and progression, but its role in oral tongue squamous cell carcinoma (OTSCC) has not yet been reported. This study aimed to investigate Beclin1 expression and its significance in OTSCC. Beclin1 expression was assessed by reverse transcriptionquantitative polymerase chain reaction or western blot analysis in 14 OTSCC tissues and matched adjacent noncancerous tissues as well as in 5 OTSCC cell lines and a normal tongue epithelial cell line. Beclin1 protein expression was examined by immunohistochemistry in 133 OTSCC specimens, and the correlation between Beclin1 expression and clinicopathological features was investigated. Furthermore, MTT and colony formation assays were performed to investigate the effect of Beclin1 on the proliferation and clonogenicity of OTSCC cells. It was demonstrated that Beclin1 expression was significantly decreased in the majority of the 14 OTSCC tissues and the 5 OTSCC cell lines relative to the matched noncancerous tissues and the normal tongue epithelial cell line, respectively. Immunohistochemistry analysis revealed that decreased Beclin1 expression was significantly correlated with poor differentiation, lymph node metastasis, advanced clinical tumournodemetastasis stage, and a poor prognosis in patients with OTSCC. The in vitro assays indicated that the overexpression of Beclin1 significantly inhibits the proliferation and clonogenicity of OTSCC cells. These results demonstrate that Beclin1 acts as a tumour suppressor in the development or progression of OTSCC and that Beclin1 may represent a novel prognostic marker for patients with OTSCC.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Língua
/
Carcinoma de Células Escamosas
/
Proteína Beclina-1
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2016
Tipo de documento:
Article