Oral delivery of dsRNA lipoplexes to German cockroach protects dsRNA from degradation and induces RNAi response.
Pest Manag Sci
; 73(5): 960-966, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-27470169
ABSTRACT
BACKGROUND:
In the past years, the concept of RNAi application for insect pest control has been proposed, considering the disruption of vital genes. However, the efficiency of RNAi is variable between different insect groups, especially by oral delivery of dsRNA. The purpose of this study is to assess the possibilities of RNAi as a tool for pest control using oral delivery of the dsRNAs encapsulated by liposome in the German cockroach Blattella germanica, which is highly sensitive to RNAi by injection of dsRNAs.RESULTS:
Injecting dsRNA into the abdomen of B. germanica caused dramatic depletion of essential α-tubulin gene and mortality. In contrast, oral delivery of the naked dsRNA resulted in lower RNAi efficiency, accounting for rapid degradation of the dsRNA in the midgut of B. germanica. Notably, we have further demonstrated that continuous ingestion of dsRNA lipoplexes in which dsRNA was encapsulated with a cationic liposome carrier was sufficient to slow down the degradation of dsRNA in the midgut and to increase the mortality of the German cockroach by significantly inhibiting α-tubulin expression in the midgut.CONCLUSION:
We provide empirical evidence that the formulation of dsRNA lipoplexes could be a plausible approach for insect pest control based on RNAi. © 2016 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Cadeia Dupla
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Portadores de Fármacos
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Blattellidae
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Estabilidade de RNA
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Interferência de RNA
Limite:
Animals
Idioma:
En
Revista:
Pest Manag Sci
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Taiwan
País de publicação:
ENGLAND
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ESCOCIA
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GB
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GREAT BRITAIN
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INGLATERRA
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REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM