Development of a microphysiological model of human kidney proximal tubule function.
Kidney Int
; 90(3): 627-37, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27521113
ABSTRACT
The kidney proximal tubule is the primary site in the nephron for excretion of waste products through a combination of active uptake and secretory processes and is also a primary target of drug-induced nephrotoxicity. Here, we describe the development and functional characterization of a 3-dimensional flow-directed human kidney proximal tubule microphysiological system. The system replicates the polarity of the proximal tubule, expresses appropriate marker proteins, exhibits biochemical and synthetic activities, as well as secretory and reabsorptive processes associated with proximal tubule function in vivo. This microphysiological system can serve as an ideal platform for ex vivo modeling of renal drug clearance and drug-induced nephrotoxicity. Additionally, this novel system can be used for preclinical screening of new chemical compounds prior to initiating human clinical trials.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eliminação Renal
/
Túbulos Renais Proximais
/
Modelos Biológicos
Limite:
Humans
Idioma:
En
Revista:
Kidney Int
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos