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Development of a microphysiological model of human kidney proximal tubule function.
Weber, Elijah J; Chapron, Alenka; Chapron, Brian D; Voellinger, Jenna L; Lidberg, Kevin A; Yeung, Catherine K; Wang, Zhican; Yamaura, Yoshiyuki; Hailey, Dale W; Neumann, Thomas; Shen, Danny D; Thummel, Kenneth E; Muczynski, Kimberly A; Himmelfarb, Jonathan; Kelly, Edward J.
Afiliação
  • Weber EJ; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Chapron A; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Chapron BD; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Voellinger JL; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Lidberg KA; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Yeung CK; Department of Pharmacy, University of Washington, Seattle, Washington, USA; Kidney Research Institute, University of Washington, Seattle, Washington, USA.
  • Wang Z; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Yamaura Y; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Hailey DW; Department of Biological Structure, University of Washington, Seattle, Washington, USA.
  • Neumann T; Nortis Inc., Seattle, Washington, USA.
  • Shen DD; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA; Department of Pharmacy, University of Washington, Seattle, Washington, USA.
  • Thummel KE; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA.
  • Muczynski KA; Department of Medicine, University of Washington, Seattle, Washington, USA.
  • Himmelfarb J; Department of Medicine, University of Washington, Seattle, Washington, USA; Kidney Research Institute, University of Washington, Seattle, Washington, USA. Electronic address: himmej@u.washington.edu.
  • Kelly EJ; Department of Pharmaceutics, University of Washington, Seattle, Washington, USA. Electronic address: edkelly@uw.edu.
Kidney Int ; 90(3): 627-37, 2016 09.
Article em En | MEDLINE | ID: mdl-27521113
ABSTRACT
The kidney proximal tubule is the primary site in the nephron for excretion of waste products through a combination of active uptake and secretory processes and is also a primary target of drug-induced nephrotoxicity. Here, we describe the development and functional characterization of a 3-dimensional flow-directed human kidney proximal tubule microphysiological system. The system replicates the polarity of the proximal tubule, expresses appropriate marker proteins, exhibits biochemical and synthetic activities, as well as secretory and reabsorptive processes associated with proximal tubule function in vivo. This microphysiological system can serve as an ideal platform for ex vivo modeling of renal drug clearance and drug-induced nephrotoxicity. Additionally, this novel system can be used for preclinical screening of new chemical compounds prior to initiating human clinical trials.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eliminação Renal / Túbulos Renais Proximais / Modelos Biológicos Limite: Humans Idioma: En Revista: Kidney Int Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eliminação Renal / Túbulos Renais Proximais / Modelos Biológicos Limite: Humans Idioma: En Revista: Kidney Int Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos