Effect of gossypol on the morphology, motility and metabolism of a flagellated protist, Dunaliella bioculata.

ABSTRACT

Gossypol, a natural polyphenolic compound extracted from cotton seed, is known for its contraceptive properties on human beings and several other mammals. Gossypol induces disorders of spermatogenesis and inhibition of spermatozoal motility. To determine the molecular mechanism inhibiting the motility of cells, a flagellar protist (Dunaliella bioculata) has been chosen as a cellular model because of the heterogeneity of spermatozoa populations. Inhibition of cell motility by gossypol, measured by Laser Doppler Velocimetry, was observed with these cells at the same doses as for spermatozoa (30-50 mumoles/l). Mitochondria were found to be swollen in gossypol-treated algae and, in parallel, respiratory exchanges were decreased and photosynthesis was inhibited, although the chloroplast did not seem to be morphologically altered. A diminution of the essential ATP production was also found at the same doses. Therefore, the inhibition of flagellar motility could be explained by such a decrease. The rapid and parallel inhibition of respiration and photosynthesis at non-lethal concentrations leads us to the hypothesis of a common target involving both ubiquinone and plastoquinone, although this remains to be proved.