Biotinylation: a simple method for labelling complement component C8 with preservation of functional activity.
J Immunol Methods
; 121(1): 61-6, 1989 Jul 06.
Article
em En
| MEDLINE
| ID: mdl-2754261
ABSTRACT
Biotinylation of human C8 with the water-soluble biotin derivative biotinylamidohexanoic acid, N-hydroxysulfosuccinimide ester is an excellent method for labelling this terminal complement component with preservation of its functional activity. The biotinylated product can be detected both in native form and also following its incorporation into the terminal complement complexes. Detection assays include Western blotting, crossed immunoblotting, ELISA, and immunocytochemistry. Biotinylation is an attractive alternative method for labelling C8 and may be used for detecting and quantifying C8 and C5b-9 complexes in their soluble and membrane-bound forms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biotina
/
Complemento C8
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1989
Tipo de documento:
Article