Synovial fluid from patients with rheumatoid arthritis modulates monocyte cell-surface phenotype.
J Int Med Res
; 44(1 suppl): 15-21, 2016 Sep.
Article
em En
| MEDLINE
| ID: mdl-27683133
ABSTRACT
OBJECTIVES:
To investigate the ability of synovial fluid from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) to modulate cell-surface phenotype, function and viability of monocytes.METHODS:
Monocytes from healthy donors were incubated with synovial fluid from patients with RA or OA. These were then cultured with autologous healthy CD4+ T-cells. Immunoglobulin-like transcript 4 (ILT4) and CD86 were evaluated on stimulated monocytes and CD4+ T-cells via fluorescence activated cell sorting.RESULTS:
Monocytes incubated with synovial fluid from patients with RA (SF-RA; n = 12) had significantly lower ILT4 and higher CD86 levels than those incubated with synovial fluid from patients with OA (SF-OA; n = 12) or medium alone. In patients with RA, there was a significant negative correlation between ILT4 and disease activity score (DAS; r = -0.699), and a positive correlation between CD86 and DAS (r = 0.626). T-cells costimulated with monocytes cultured with SF-RA produced significantly more interferon-γ and tumour necrosis factor-α than those costimulated with monocytes cultured with SF-OA or controls.CONCLUSIONS:
Soluble mediators in SF-RA could contribute to modulating inflammation and local effectiveness of the immune response.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Int Med Res
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Itália