Subunit analysis of mitochondrial cytochrome c oxidase and cytochrome bc1 by reversed-phase high-performance liquid chromatography.
Anal Biochem
; 516: 6-8, 2017 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-27720837
ABSTRACT
A rapid separation of the ten nuclearly-encoded subunits of mitochondrial cytochrome c oxidase, and ten out of the eleven subunits of cytochrome bc1, was achieved using a short, 50 mm C18-reversed-phase column. The short column decreased the elution time 4-7 fold while maintaining the same resolution quality. Elution was similar to a previously published protocol, i.e., a water/acetonitrile elution gradient containing trifluoroacetic acid. Isolated subunits were identified by MALDI-TOF. The rapidity of the described method makes it extremely useful for determining the subunit composition of isolated mitochondrial complexes. The method can be used for both analytical and micro-preparative purposes.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complexo IV da Cadeia de Transporte de Elétrons
/
Complexo III da Cadeia de Transporte de Elétrons
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Proteínas Mitocondriais
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Cromatografia de Fase Reversa
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Mitocôndrias Cardíacas
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2017
Tipo de documento:
Article