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The development of methods for the detection of Salmonella in chickens by a combination of immunomagnetic separation and PCRs.
Biotechnol Appl Biochem ; 64(6): 888-894, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27730684
ABSTRACT
Micro- and nanoimmunomagnetic beads (MIMBs and NIMBs) used for immunomagnetic separation (IMS) with PCR were studied for the rapid detection of Salmonella. The capture efficiency of the two different IMBs was evaluated by a conventional plate counting method, and the binding pattern was studied using scanning electron microscopy. The specificity of the IMBs was tested with Salmonella, Shigella flexneri, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogenes. By comparing the pre-enrichment IMS and the IMS enrichment steps with a 5.5-H enrichment time, this study developed a rapid and sensitive method for the detection of Salmonella in chicken. The method was implemented by IMS enrichment and PCR with MIMBs and NIMBs, with a total analysis time of 8 H. We showed that the method was sensitive based on NIMBs with a detection limit of 10° CFU for Salmonella in 25 g of chicken.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Salmonella / Reação em Cadeia da Polimerase / Separação Imunomagnética Limite: Animais Idioma: Inglês Revista: Biotechnol Appl Biochem Assunto da revista: Bioquímica / Biotecnologia Ano de publicação: 2017 Tipo de documento: Artigo