Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).
Arch Virol
; 162(2): 543-548, 2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-27804019
ABSTRACT
To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
RNA Viral
/
Síndrome de Imunodeficiência Adquirida dos Símios
/
Vírus da Imunodeficiência Símia
/
Cercopithecinae
Limite:
Animals
/
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Japão