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Inactivation of Middle East respiratory syndrome coronavirus (MERS-CoV) in plasma products using a riboflavin-based and ultraviolet light-based photochemical treatment.
Keil, Shawn D; Bowen, Richard; Marschner, Susanne.
Afiliação
  • Keil SD; Terumo BCT, Lakewood, Colorado.
  • Bowen R; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
  • Marschner S; Terumo BCT, Lakewood, Colorado.
Transfusion ; 56(12): 2948-2952, 2016 12.
Article em En | MEDLINE | ID: mdl-27805261
ABSTRACT

BACKGROUND:

Middle East respiratory syndrome coronavirus (MERS-CoV) has been identified as a potential threat to the safety of blood products. The Mirasol Pathogen Reduction Technology System uses riboflavin and ultraviolet (UV) light to render blood-borne pathogens noninfectious while maintaining blood product quality. Here, we report on the efficacy of riboflavin and UV light against MERS-CoV when tested in human plasma. STUDY DESIGN AND

METHODS:

MERS-CoV (EMC strain) was used to inoculate plasma units that then underwent treatment with riboflavin and UV light. The infectious titers of MERS-CoV in the samples before and after treatment were determined by plaque assay on Vero cells. The treatments were initially performed in triplicate using pooled plasma (n = 3) and then repeated using individual plasma units (n = 6).

RESULTS:

In both studies, riboflavin and UV light reduced the infectious titer of MERS-CoV below the limit of detection. The mean log reductions in the viral titers were ≥4.07 and ≥4.42 for the pooled and individual donor plasma, respectively.

CONCLUSION:

Riboflavin and UV light effectively reduced the titer of MERS-CoV in human plasma products to below the limit of detection, suggesting that the treatment process may reduce the risk of transfusion transmission of MERS-CoV.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inativação de Vírus / Segurança do Sangue / Coronavírus da Síndrome Respiratória do Oriente Médio Limite: Animals / Humans Idioma: En Revista: Transfusion Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inativação de Vírus / Segurança do Sangue / Coronavírus da Síndrome Respiratória do Oriente Médio Limite: Animals / Humans Idioma: En Revista: Transfusion Ano de publicação: 2016 Tipo de documento: Article