Cannabidiol (CBD) induces functional Tregs in response to low-level T cell activation.
Cell Immunol
; 312: 25-34, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-27865421
ABSTRACT
Many effects of the non-psychoactive cannabinoid, cannabidiol (CBD), have been described in immune responses induced by strong immunological stimuli. It has also been shown that CBD enhances IL-2 production in response to low-level T cell stimulation. Since IL-2, in combination with TGF-ß1, are critical for Treg induction, we hypothesized that CBD would induce CD4+CD25+FOXP3+ Tregs in response to low-level stimulation. Low-level T cell stimulation conditions were established based on minimal CD25 expression in CD4+ cells using suboptimal PMA/Io (4nM/0.05µM, S/o), ultrasuboptimal PMA/Io (1nM/0.0125µM, Us/o) or soluble anti-CD3/28 (400-800ng each, s3/28). CBD increased CD25+FOXP3+ cells from CD4+, CD4+CD25+, and CD4+CD25- T cells, as well as in CD4+ T cells derived from FOXP3-GFP mice. Most importantly, the Us/o+CBD-induced CD4+CD25+ Tregs robustly suppressed responder T cell proliferation, demonstrating that the mechanism by which CBD is immunosuppressive under low-level T cell stimulation involves induction of functional Tregs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canabidiol
/
Linfócitos T Reguladores
/
Imunossupressores
Limite:
Animals
Idioma:
En
Revista:
Cell Immunol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos