13 Câ
MR Hyperpolarization of Lactate by Using ParaHydrogen and Metabolic Transformation in Vitro.
Chemistry
; 23(5): 1200-1204, 2017 Jan 23.
Article
em En
| MEDLINE
| ID: mdl-27870463
ABSTRACT
Hyperpolarization of the 13 C magnetic resonance signal of l-[1-13 C]lactate has been obtained using the chemically based, cost-effective method called parahydrogen-induced polarization by means of side-arm hydrogenation (PHIP-SAH). Two ester derivatives of lactate were tested and the factors that determine the polarization level on the product have been investigated in detail. The metabolic conversion of hyperpolarized l-[1-13 C]lactate into pyruvate has been observed in vitro using lactate dehydrogenase (LDH) and in a cells lysate. From the acquisition of a series of 13 Câ
NMR spectra, the metabolic build-up of the [1-13 C]pyruvate signal has been observed. These studies demonstrate that, even if the experimental set-up used for these PHIP-SAH hyperpolarization studies is still far from optimal, the attained polarization level is already sufficient to carry out in vitro metabolic studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
/
Hidrogênio
Idioma:
En
Revista:
Chemistry
Assunto da revista:
QUIMICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Itália