ß-Galactosidase Langmuir Monolayer at Air/X-gal Subphase Interface.
J Phys Chem B
; 120(48): 12279-12286, 2016 12 08.
Article
em En
| MEDLINE
| ID: mdl-27934226
ABSTRACT
This article investigates the surface chemistry properties of the ß-galactosidase monolayer at the air-subphase interface at the vicinity of its substrate, X-gal. We have demonstrated that the ß-galactosidase in the monolayer form remained active and performed hydrolysis of the X-gal in the subphase. We investigated the ß-galactosidase Langmuir monolayer in absence and presence of X-gal in the subphase of varying concentration of X-gal with the sodium chloride solution. It was found that the limiting molecular area as well as the collapse surface pressure kept on decreasing with the increasing concentration of X-gal. In accordance to the data obtained from the isotherm it was also found that ß-galactosidase forms a stable monolayer that does not aggregate at the air-subphase interface. The stability of the monolayer at the air-subphase interface was studied by using compression-decompression cycles with and without X-gal at varying concentration and different surface pressures. The infrared reflection-absorption spectroscopy (IRRAS) and Brewster angle microscopy (BAM) of ß-galactosidase Langmuir monolayer was also investigated for pure and mixed ß-galactosidase at the air-subphase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Galactosidase
/
Galactosídeos
/
Indóis
Idioma:
En
Revista:
J Phys Chem B
Assunto da revista:
QUIMICA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos