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BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions.
Orth, M; Kruse, N J; Braun, B J; Scheuer, C; Holstein, J H; Khalil, A; Yu, X; Murphy, W L; Pohlemann, T; Laschke, M W; Menger, M D.
Afiliação
  • Orth M; Department of Trauma, Hand and Reconstructive Surgery, Saarland University, Kirrberger Strasse 1, D-66421 Homburg/Saar, Germany.marcel.orth@uks.eu.
Eur Cell Mater ; 33: 1-12, 2017 01 02.
Article em En | MEDLINE | ID: mdl-28054333
Atrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to apply Bone Morphogenetic Protein-2 (BMP-2) to improve bone repair of atrophic non-unions. For this purpose, atrophic non-unions were induced in femurs of CD-1 mice (n = 48). Animals either received BMP-2-coated MCM (MCM + BMP; n = 16), uncoated MCM (MCM; n = 16) or no MCM (NONE; n = 16). Bone healing was evaluated 2 and 10 weeks postoperatively by micro-computed tomographic (µCT), biomechanical, histomorphometric and immunohistochemical analyses. µCT revealed more bone volume with more highly mineralised bone in MCM + BMP femurs. Femurs of MCM + BMP animals showed a significantly higher bending stiffness compared to other groups. Histomorphometry further demonstrated that the callus of MCM + BMP femurs was larger and contained more bone and less fibrous tissue. After 10 weeks, 7 of 8 MCM + BMP femurs presented with complete osseous bridging, whereas NONE femurs exhibited a non-union rate of 100 %. Of interest, immunohistochemistry could not detect macrophages within the callus, indicating a good biocompatibility of MCM. In conclusion, the local application of BMP-2-coated MCM improved bone healing in a challenging murine non-union model and, thus, should be of clinical interest in the treatment of non-unions.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Consolidação da Fratura / Materiais Revestidos Biocompatíveis / Proteína Morfogenética Óssea 2 / Fraturas não Consolidadas / Microesferas / Minerais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Eur Cell Mater Ano de publicação: 2017 Tipo de documento: Article País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Consolidação da Fratura / Materiais Revestidos Biocompatíveis / Proteína Morfogenética Óssea 2 / Fraturas não Consolidadas / Microesferas / Minerais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Eur Cell Mater Ano de publicação: 2017 Tipo de documento: Article País de publicação: Suíça