Chemokinetic effects of exogenous and endogenous tumor necrosis factor alpha on human blood monocytes.
Int Arch Allergy Appl Immunol
; 90(1): 11-5, 1989.
Article
em En
| MEDLINE
| ID: mdl-2807575
ABSTRACT
The possible chemokinetic effect of human tumor necrosis factor alpha (TNF-alpha) upon human monocytes was investigated by using a type 1 collagen gel system. Human recombinant TNF-alpha was found to be chemokinetic, and concentrations of that cytokine as low as 0.7 pg/microliters enhanced the movement of those leukocytes. Furthermore, we explored whether endogenous TNF-alpha stimulates monocyte movement by incubating blood monocytes with a TNF-alpha-stimulating agent, human recombinant interleukin 1, with or without antibodies to human TNF-alpha. Human recombinant interleukin 1 enhanced the motility of blood monocytes, and that effect was blocked by rabbit polyclonal antibodies to human TNF-alpha. Furthermore, the movement of blood monocytes no exposed to exogenous cytokines was decreased after incubation with anti-TNF-alpha. Thus, endogenous as well as exogenous TNF-alpha is a chemokinetic agent for human monocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
/
Fatores Quimiotáticos
/
Quimiotaxia de Leucócito
/
Fator de Necrose Tumoral alfa
Limite:
Adult
/
Animals
/
Humans
Idioma:
En
Revista:
Int Arch Allergy Appl Immunol
Ano de publicação:
1989
Tipo de documento:
Article