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Imaging proteins at the single-molecule level.
Proc Natl Acad Sci U S A ; 114(7): 1474-1479, 2017 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-28087691
Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process.





Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Holografia / Proteínas / Imagem Individual de Molécula Limite: Animais Idioma: Inglês Revista: Proc Natl Acad Sci U S A Ano de publicação: 2017 Tipo de documento: Artigo