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An Aptamer-based Sensor for Unchelated Gadolinium(III).
Edogun, Osafanmwen; Chan, Tracy Y; Nguyen, Nghia H; Luu, Anthony; Halim, Marlin.
Afiliação
  • Edogun O; Department of Chemistry and Biochemistry, California State University, East Bay.
  • Chan TY; Department of Chemistry and Biochemistry, California State University, East Bay.
  • Nguyen NH; Department of Chemistry and Biochemistry, California State University, East Bay.
  • Luu A; Department of Chemistry and Biochemistry, California State University, East Bay.
  • Halim M; Department of Chemistry and Biochemistry, California State University, East Bay; marlin.halim@csueastbay.edu.
J Vis Exp ; (119)2017 01 09.
Article em En | MEDLINE | ID: mdl-28117790
A method for determining the presence of unchelated trivalent gadolinium ion (Gd3+) in aqueous solution is demonstrated. Gd3+ is often present in samples of gadolinium-based contrast agents as a result of incomplete reactions between the ligand and the ion, or as a dissociation product. Since the ion is toxic, its detection is of critical importance. Herein, the design and usage of an aptamer-based sensor (Gd-sensor) for Gd3+ are described. The sensor produces a fluorescence change in response to increasing concentrations of the ion, and has a limit of detection in the nanomolar range (~100 nM with a signal-to-noise ratio of 3). The assay may be run in an aqueous buffer at ambient pH (~7 - 7.4) in a 384-well microplate. The sensor is relatively unreactive toward other physiologically relevant metal ions such as sodium, potassium, and calcium ions, although it is not specific for Gd3+ over other trivalent lanthanides such as europium(III) and terbium(III). Nevertheless, the lanthanides are not commonly found in contrast agents or the biological systems, and the sensor may therefore be used to selectively determine unchelated Gd3+ in aqueous conditions.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Eletrodos Seletivos de Íons / Aptâmeros de Nucleotídeos / Gadolínio Idioma: En Revista: J Vis Exp Ano de publicação: 2017 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Eletrodos Seletivos de Íons / Aptâmeros de Nucleotídeos / Gadolínio Idioma: En Revista: J Vis Exp Ano de publicação: 2017 Tipo de documento: Article País de publicação: Estados Unidos