Hydrodynamic instability of "stroma-free" hemoglobin.
Biomater Artif Cells Artif Organs
; 17(3): 335-9, 1989.
Article
em En
| MEDLINE
| ID: mdl-2819259
ABSTRACT
A simple kinetic test with visual observation of hemoglobin solutions under 4 - 10x magnification was used to detect and roughly characterize a rapid formation of fine fibrous inhomogeneities in agitated "stroma-free" hemolyzates (SFH). In parallel SFH samples stored motionless for months, no such precipitate was observed. Hydrodynamic conditions are necessary to provoke a stepwise aggregation of small amounts of unstable filamentous nonhemoglobin molecules originating mostly from the stromata of erythrocytes and from constituents of other lysed blood cells. Numerous screening experiments mentioned here failed to remove significantly the "fiber-forming" substances from SFH or to prevent their precipitation. Development of a hydrodynamically stable and better purified SFH seems to be a prerequisite for further progress in the field of infusable SFH and its chemically modified variants (MSFH).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hemoglobinas
Limite:
Humans
Idioma:
En
Revista:
Biomater Artif Cells Artif Organs
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
/
TRANSPLANTE
Ano de publicação:
1989
Tipo de documento:
Article