An experimental study of a rat model of emphysema induced by cigarette smoke exposure and the effect of Survanta therapy.
Ann Anat
; 211: 69-77, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-28199823
ABSTRACT
The present study was performed to test the therapeutic effects of Survanta (an exogenous surfactant) on a Wistar rat model of emphysema. Thirty-five adult male Wistar rats were divided randomly into the following groups; control subgroups Ia&b (n=14); emphysematous model subgroups IIa,b&c (n=21) exposed to cigarette smoke (CS), received phosphate buffer solution (PBS) and Survanta respectively. The levels of serum myeloperoxidase (MPO), lung tissue lactate dehydrogenase (LDH), alkaline phosphatase (ALP) as well as antioxidants catalase (CAT), superoxide dismutase (SOD) and oxidative stress malondialdehyde (MDA) markers were measured. Immunohistochemical staining of the lung was applied with anti-P53, anti- tumor necrosis factor (TNFα) and anti-proliferating cell nuclear antigen (PCNA) to reveal the changes of the lung structure. The mean linear intercepts (MLI) of alveoli were measured to assess alveolar size. In emphysematous rats, the serum level of MPO and tissue LDH, ALP & MDA were significantly increased while; CAT and SOD were significantly decreased. Pictures analysis for all immunostains was clearly increased. In Survanta treated group, a significant improvement in all previously mentioned findings while; no improvement in alveolar diameter was detected. These results conclusively demonstrate that Survanta administration improves the inflammatory biochemical and histochemical parameters of the emphysematous lung.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Enfisema Pulmonar
/
Produtos Biológicos
/
Fumar
/
Modelos Animais de Doenças
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Ann Anat
Assunto da revista:
ANATOMIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Egito