The lactoferricin B-derived peptide, LfB17-34, induces melanogenesis in B16F10 cells.
Int J Mol Med
; 39(3): 595-602, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-28204812
ABSTRACT
Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immunomodulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this study, a set of five LfcinB-related peptides was examined. We found that LfB1734, an 18-mer LfcinB-derived peptide, increased melanogenesis in B16F10 melanoma cells without significantly affecting cell viability. LfB1734 increased in vitro tyrosinase activity and melanin content in a dose-dependent manner. The results of RT-qPCR and western blot analyses showed that LfB1734 increased the mRNA and protein expression of tyrosinase and tyrosinase-related protein 1 (Trp1). Moreover, LfB1734 inhibited the phosphorylation of MAPK/Erk, but not p38 and Akt, and constitutively active MEK was able to reverse the LfB17-34-enhanced pigmentation, melanin content, and tyrosinase activity, suggesting a role of Erk signaling in the process of LfB1734-mediated pigmentation. Taken together, these results suggest that LfB1734 induces melanogenesis in B16F10 cells primarily through increased tyrosinase expression and activity and that LfB1734 could be further developed for the treatment of hypopigmentation disorders.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Transformação Celular Neoplásica
/
Lactoferrina
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2017
Tipo de documento:
Article