Reversible Light-Switching of Enzymatic Activity on Orthogonally Functionalized Polymer Brushes.
ACS Appl Mater Interfaces
; 9(11): 9245-9249, 2017 Mar 22.
Article
em En
| MEDLINE
| ID: mdl-28266210
ABSTRACT
Copolymer brushes, composed of glycidyl methacrylate and a furan-protected maleimide-containing monomer, were grafted from radical initiators at the surface of irradiation-activated fluoropolymer foils. After postpolymerization modification with enzymatically active microperoxidase-11 and photochromic spiropyran moieties, the polymer brushes catalyzed the oxidation of 3,3'5,5'-tetramethylbenzidine. Exposure to either UV or visible-light allowed switching the turnover by more than 1 order of magnitude, as consequence of the reversible, light-induced spiropyran-merocyanine transition. The modified samples were integrated into an optofluidic device that allowed the reversible switching of enzymatic activity for several cycles under flow, validating the potential for application in smart lab-on-a-chip systems.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Ano de publicação:
2017
Tipo de documento:
Article
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