Recombinant expression of porcine spermadhesin AWN and its phospholipid interaction: Indication for a novel lipid binding property.
Reprod Domest Anim
; 52(4): 585-595, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28326614
ABSTRACT
AWN is a porcine (Sus scrofa domestica) seminal plasma protein and has been linked to a variety of processes related to fertilization. To acquire the protein in sufficient amount and purity for functional studies, we established its recombinant expression in E. coli and a three-step purification protocol based on different chromatographies. The test for AWN-phospholipid interaction revealed phosphatidic acid and cardiolipin as potential binding partners. As phosphatidic acid is surmised to play a role in cation-induced membrane destabilization and fusion events, we propose a membrane protective function of the presented binding affinity. Further studies with recombinant AWN will allow new insights into the mechanism of sperm-spermadhesin interaction and might provide new approaches for artificial reproduction techniques.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Proteínas Recombinantes
/
Proteínas de Transporte
/
Proteínas de Plasma Seminal
Limite:
Animals
Idioma:
En
Revista:
Reprod Domest Anim
Assunto da revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha