Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression.
Braz J Infect Dis
; 21(3): 248-254, 2017.
Article
em En
| MEDLINE
| ID: mdl-28347732
ABSTRACT
OBJECTIVE:
To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression.METHODS:
We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups positive (n=75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n=52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n=48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).RESULTS:
The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p=0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p<0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio 2.633; 95% CI, 1.399-4.954, p=0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups.CONCLUSION:
The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
RNA Mensageiro
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Displasia do Colo do Útero
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Neoplasias do Colo do Útero
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Proteínas Oncogênicas Virais
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Proteína Supressora de Tumor p53
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Infecções por Papillomavirus
/
Proteínas E7 de Papillomavirus
Tipo de estudo:
Risk_factors_studies
Limite:
Adolescent
/
Adult
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Aged
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Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Braz J Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2017
Tipo de documento:
Article