An Open-Source Automated Peptide Synthesizer Based on Arduino and Python.
SLAS Technol
; 22(5): 493-499, 2017 10.
Article
em En
| MEDLINE
| ID: mdl-28378612
ABSTRACT
The development of the first open-source automated peptide synthesizer, PepSy, using Arduino UNO and readily available components is reported. PepSy was primarily designed to synthesize small peptides in a relatively small scale (<100 µmol). Scripts to operate PepSy in a fully automatic or manual mode were written in Python. Fully automatic script includes functions to carry out resin swelling, resin washing, single coupling, double coupling, Fmoc deprotection, ivDde deprotection, on-resin oxidation, end capping, and amino acid/reagent line cleaning. Several small peptides and peptide conjugates were successfully synthesized on PepSy with reasonably good yields and purity depending on the complexity of the peptide.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Técnicas de Química Sintética
Idioma:
En
Revista:
SLAS Technol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos