High-resolution myogenic lineage mapping by single-cell mass cytometry.
Nat Cell Biol
; 19(5): 558-567, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28414312
ABSTRACT
Muscle regeneration is a dynamic process during which cell state and identity change over time. A major roadblock has been a lack of tools to resolve a myogenic progression in vivo. Here we capitalize on a transformative technology, single-cell mass cytometry (CyTOF), to identify in vivo skeletal muscle stem cell and previously unrecognized progenitor populations that precede differentiation. We discovered two cell surface markers, CD9 and CD104, whose combined expression enabled in vivo identification and prospective isolation of stem and progenitor cells. Data analysis using the X-shift algorithm paired with single-cell force-directed layout visualization defined a molecular signature of the activated stem cell state (CD44+/CD98+/MyoD+) and delineated a myogenic trajectory during recovery from acute muscle injury. Our studies uncover the dynamics of skeletal muscle regeneration in vivo and pave the way for the elucidation of the regulatory networks that underlie cell-state transitions in muscle diseases and ageing.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração
/
Células-Tronco
/
Separação Celular
/
Músculo Esquelético
/
Linhagem da Célula
/
Desenvolvimento Muscular
/
Mioblastos Esqueléticos
/
Análise de Célula Única
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Citometria de Fluxo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Nat Cell Biol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos