[Physical mapping of the plasmid R205 and identification of a site responsible for increased UV-induced mutability of the bacterial host]. / Fizicheskoe kartirovanie plazmidy R205 i identifikatsiia raiona, otvetstvennogo za povyshenie UF-indutsiruemoi mutabil'nosti bakterii-khoziaina.

ABSTRACT

A physical map of the conjugative IncN plasmid R205 (56.1 kb) was constructed. The distribution of cleavage sites for investigated restriction enzymes is asymmetric. It was found that R205 suppresses the mutant phenotype of E. coli K12 umuC or umuD strains deficient in UV-induced mutagenesis. A mini-derivative of R205, designated pMU4 (15.1 kb) preserves the ability of the parent plasmid to increase the survival and induced mutagenesis of UV-irradiated host cells. A region of R205 located between 0 and 2.0 kb-on the plasmid map seems to contain information necessary for complementation of mutation in the host genes umuD/C, Hybridization between this region of pMU4 and plasmid pGW1700 bearing mucAB genes of pKM101 was observed.