Synthesis of human rotavirus polypeptides in cell culture.
J Med Virol
; 25(1): 91-103, 1988 May.
Article
em En
| MEDLINE
| ID: mdl-2842450
ABSTRACT
The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of infection of the virus, incorporation of actinomycin D and excess NaCl into the medium resulted in significant reduction in host cell background and enabled observation of viral polypeptides as early as 2 hours post infection. Five polypeptides were found to be structural components of the virion, and a further eight appeared to be nonstructural proteins or intermediates. Five polypeptides were glycosylated during virus replication, but only one of these, VP7, was a definite structural glycoprotein. Pulse-chase experiments revealed that four low molecular weight polypeptides underwent post-translational modifications.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese Peptídica
/
Proteínas Virais
/
Rotavirus
Limite:
Animals
Idioma:
En
Revista:
J Med Virol
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Austrália