Inducible expression of herpes simplex virus type 2 glycoprotein gene gG-2 in a mammalian cell line.
J Virol
; 62(10): 3668-74, 1988 Oct.
Article
em En
| MEDLINE
| ID: mdl-2843667
ABSTRACT
The gG-2 glycoprotein gene of herpes simplex virus type 2 (HSV-2) was cloned into the mammalian expression vector pMSG under the control of the inducible mouse mammary tumor virus promoter. Transfection of this cloned gG-2 construct into NIH 3T3 cells resulted in the stable expression of gG-2 upon induction with dexamethasone. In addition, the 104,000-molecular-weight (104K) and 72K gG-2 precursors as well as the 34K secreted component were generated in the transformed cells. The synthesis of gG-2 in these transformed cells appeared to follow the same cleavage-processing pathway as gG-2 synthesis during an HSV-2 infection. These results indicate that the processing of gG-2 can occur in the absence of an HSV-2 infection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Envelope Viral
/
Regulação da Expressão Gênica
/
Simplexvirus
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
1988
Tipo de documento:
Article