TGF-ß1 improving abnormal pregnancy outcomes induced by Toxoplasma gondii infection: Regulating NKG2D/DAP10 and killer subset of decidual NK cells.

ABSTRACT

Our current aim was to investigate whether injection of TGF-ß1 played an important role in improving abnormal pregnancy outcomes with T. gondii infection and how the TGF-ß1 regulated. Results showed that TGF-ß1 exhibited improved pregnancy outcomes induced by T. gondii infection. dNK cytotoxicity was increased with T. gondii infection while decreased with TGF-ß1 treatment. dNK cytotoxicity related NKG2D/DAP10 expression, perforin, granzyme, IFN-γ and killer subsets were all increased with T. gondii infection while decreased after TGF-ß1 treatment. In addition, anti-TGF-ß1 antibodies could aggregate the cytotoxicity of dNK cells and the levels of molecules above. These results indicated that TGF-ß1 treatment could improve the abnormal pregnancy outcomes with T. gondii infection by decreasing the cytotoxicity of dNK cells mediated by NKG2D/DAP10 pathway and killer subset. These results suggested that TGF-ß1 might be a potential immunoprotective method for the treatment of abnormal pregnancy outcomes following T. gondii infection.