Rifampin and rpoB mutations can alter DNA supercoiling in Escherichia coli.
J Bacteriol
; 170(10): 4983-5, 1988 Oct.
Article
em En
| MEDLINE
| ID: mdl-2844734
ABSTRACT
Two cases are described which indicate that RNA polymerase could alter DNA supercoiling. One occurred in a topA mutant in which abnormally high levels of plasmid supercoiling were lowered by rifampin, an inhibitor of the beta subunit of RNA polymerase. The second case involves suppression of a temperature-sensitive gyrB mutation by a rifampin-resistant allele of rpoB, the gene encoding the beta subunit of RNA polymerase. Measurements of chromosomal DNA supercoiling show that the rpoB mutation reduced DNA relaxation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rifampina
/
RNA Polimerases Dirigidas por DNA
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DNA Bacteriano
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DNA Super-Helicoidal
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Escherichia coli
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1988
Tipo de documento:
Article