Construction of ferrocene modified conducting polymer based amperometric urea biosensor.
Enzyme Microb Technol
; 102: 53-59, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28465061
ABSTRACT
Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor's amperometric response was linear in the range of 0.1-8.5mM with the sensitivity of 0.54µA/mM, detection limit of 12µM, and short response time of 2s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insignificant interference.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ureia
/
Compostos Ferrosos
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Técnicas Biossensoriais
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Metalocenos
Limite:
Humans
Idioma:
En
Revista:
Enzyme Microb Technol
Ano de publicação:
2017
Tipo de documento:
Article