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A conserved regulatory mechanism in bifunctional biotin protein ligases.
Wang, Jingheng; Beckett, Dorothy.
Afiliação
  • Wang J; Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, 20742.
  • Beckett D; Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, 20742.
Protein Sci ; 26(8): 1564-1573, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28466579
ABSTRACT
Class II bifunctional biotin protein ligases (BirA), which catalyze post-translational biotinylation and repress transcription initiation, are broadly distributed in eubacteria and archaea. However, it is unclear if these proteins all share the same molecular mechanism of transcription regulation. In Escherichia coli the corepressor biotinoyl-5'-AMP (bio-5'-AMP), which is also the intermediate in biotin transfer, promotes operator binding and resulting transcription repression by enhancing BirA dimerization. Like E. coli BirA (EcBirA), Staphylococcus aureus, and Bacillus subtilis BirA (Sa and BsBirA) repress transcription in vivo in a biotin-dependent manner. In this work, sedimentation equilibrium measurements were performed to investigate the molecular basis of this biotin-responsive transcription regulation. The results reveal that, as observed for EcBirA, Sa, and BsBirA dimerization reactions are significantly enhanced by bio-5'-AMP binding. Thus, the molecular mechanism of the Biotin Regulatory System is conserved in the biotin repressors from these three organisms.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Staphylococcus aureus / Bacillus subtilis / Transcrição Gênica / Biotina / Monofosfato de Adenosina / Carbono-Nitrogênio Ligases / Proteínas de Escherichia coli / Escherichia coli Tipo de estudo: Prognostic_studies Idioma: En Revista: Protein Sci Assunto da revista: BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Staphylococcus aureus / Bacillus subtilis / Transcrição Gênica / Biotina / Monofosfato de Adenosina / Carbono-Nitrogênio Ligases / Proteínas de Escherichia coli / Escherichia coli Tipo de estudo: Prognostic_studies Idioma: En Revista: Protein Sci Assunto da revista: BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article