Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains.
Vaccine
; 35(24): 3204-3208, 2017 05 31.
Article
em En
| MEDLINE
| ID: mdl-28479173
ABSTRACT
Previously, we constructed and characterized the vaccine efficacy of Salmonella Typhimurium mutant strains in poultry with either inducible mviN expression (PBAD-mviN) or methionine auxotrophy (ΔΔmetRmetD). The aim of the present study was to assess potential impact of these Salmonella vaccine strains on the cecal microbiota using a next generation sequencing (NGS). The cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with PBAD-mviN; group 3, vaccinated with wild type; group 4, vaccinated with ΔΔmetRmetD) were subjected to microbiome sequencing analysis with an Illumina MiSeq platform. The NGS microbiome analysis of chicken ceca revealed considerable changes in microbial composition in the presence of the different vaccine strains and exhibited detectable patterns of distinctive clustering among the respective groups (the R value of unweighted PCoA plot was 0.68). The present study indicates that different S. Typhimurium vaccine strains can differentially influence the microbiota of the ceca in terms of presence but not in the relative abundance of microbiota.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ceco
/
Galinhas
/
Vacinas contra Salmonella
/
Microbioma Gastrointestinal
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos