Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals.

Vargas, S L; Ponce, C; Bustamante, R; Calderón, E; Nevez, G; De Armas, Y; Matos, O; Miller, R F; Gallo, M J

Vargas, S L; Ponce, C; Bustamante, R; Calderón, E; Nevez, G; De Armas, Y; Matos, O; Miller, R F; Gallo, M J.

Afiliação

Vargas SL; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, 8380453, Santiago, Chile. svargas@med.uchile.cl.
Ponce C; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, 8380453, Santiago, Chile.
Bustamante R; Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, 8380453, Santiago, Chile.
Calderón E; Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP) and Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
Nevez G; Laboratory of Parasitology and Mycology, Brest University Hospital, & University of Brest, GEIHP, EA 3142, Brest, France.
De Armas Y; Hospital Microbiology Department, Institute of Tropical Medicine "Pedro Kourí" Pathology Department, Institute of Tropical Medicine "Pedro Kourí" Hospital, Havana, Cuba.
Matos O; Unidade de Parasitología Médica, Grupo de Protozoários Oportunistas/VIH e Outros Protozoarios, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, 1349-008, Lisbon, Portugal.
Miller RF; Research Department of Infection and Population Health, Institute of Global Health, University College London, Mortimer Market Street, London, WC1E 6BT, UK.
Gallo MJ; Clinical Research Department, London School of Hygiene and Tropical Medicine, London, UK.

Eur J Clin Microbiol Infect Dis ; 36(10): 1711-1716, 2017 Oct.

Article em En | MEDLINE | ID: mdl-28584896

ABSTRACT

ABSTRACT

To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.

Assuntos

Autopsia/métodos; Pulmão/microbiologia; Técnicas Microbiológicas/métodos; Pneumocystis/isolamento & purificação; Pneumonia por Pneumocystis/diagnóstico; Manejo de Espécimes/métodos; Humanos; Pneumonia por Pneumocystis/microbiologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumocystis / Pneumonia por Pneumocystis / Manejo de Espécimes / Autopsia / Técnicas Microbiológicas / Pulmão Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile

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