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Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis.
Kanagavel, Murugesan; Shanmughapriya, Santhanam; Aishwarya, Kayanam Vijaya Lalitha; Ponmurugan, Karuppiah; Murugan, Kasi; Al-Dhabi, Naif Abdullah; Natarajaseenivasan, Kalimuthusamy.
Afiliação
  • Kanagavel M; Medical Microbiology Laboratory, Department of Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, TN, 620024, India.
  • Shanmughapriya S; Medical Microbiology Laboratory, Department of Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, TN, 620024, India.
  • Aishwarya KVL; Center for Translational Medicine, School of Medicine, Temple University, Philadelphia, PA, 19140, USA.
  • Ponmurugan K; Medical Microbiology Laboratory, Department of Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, TN, 620024, India.
  • Murugan K; Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
  • Al-Dhabi NA; Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
  • Natarajaseenivasan K; Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, TN, 627012, India.
Sci Rep ; 7(1): 3250, 2017 06 12.
Article em En | MEDLINE | ID: mdl-28607384
Leptospirosis is underdiagnosed due to low sensitivity, need of specialised equipment, and expensive reagents for serological and molecular diagnosis respectively. Considering the sensitivity, rapidity, inexpensive reagents and collection of clinical samples, the monoclonal antibody based antigen detection method from urine samples has been developed and evaluated. LigA (LK90) based B-cell specific epitopes were predicted and synthesised as peptides for the production of monoclonal antibody. LK90543: SNAQKNQGNA (amino acids: 543 to 552), and LK901110: DHHTQSSYTP (amino acids: 1110 to 1119) with VaxiJen score of 1.3719 and 1.2215, respectively were used. Thirty two and 28 urine samples from confirmed and seronegative healthy human subjects, respectively were included for the evaluation of MAb-based dot blot ELISA. The specificity of the evaluated MAbs, P1B1 and P4W2 were found to be in the range of ~93-96%. Moreover, the MAbs did not show cross-reactivity with other bacterial antigens as confirmed by IgG ELISA, further validating its specificity for leptospiral antigens. These findings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than 8 h, inexpensive with a ICER of $8.7/QALY, and affordable in developing countries and area where laboratory facilities are limited.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Leptospira / Leptospirose / Anticorpos Monoclonais Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adolescent / Adult / Animals / Child / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Índia País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Leptospira / Leptospirose / Anticorpos Monoclonais Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adolescent / Adult / Animals / Child / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Índia País de publicação: Reino Unido