Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut.
Biochemistry
; 56(30): 3889-3893, 2017 08 01.
Article
em En
| MEDLINE
| ID: mdl-28682052
ABSTRACT
The diverse gut microbial communities are crucial for host health. How the interactions between microbial communities and between host and microbes influence the host, however, is not well understood. To facilitate gut microbiota research, selective imaging of specific groups of microbiotas in the gut is of great utility but remains technically challenging. Here we present a chemical approach that enables selective imaging of Gram-negative and Gram-positive microbiotas in the mouse gut by exploiting their distinctive cell wall components. Cell-selective labeling is achieved by the combined use of metabolic labeling of Gram-negative bacterial lipopolysaccharides with a clickable azidosugar and direct labeling of Gram-positive bacteria with a vancomycin-derivatized fluorescent probe. We demonstrated this strategy by two-color fluorescence imaging of Gram-negative and Gram-positive gut microbiotas in the mouse intestines. This chemical method should be broadly applicable to different gut microbiota research fields and other bacterial communities studied in microbiology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trato Gastrointestinal
/
Técnicas de Diagnóstico do Sistema Digestório
/
Disbiose
/
Microbioma Gastrointestinal
/
Bactérias Gram-Negativas
/
Bactérias Gram-Positivas
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China