Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay.
Virology
; 509: 260-265, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-28688346
ABSTRACT
Bovine viral diarrhea virus (BVDV) infections, whether as acute, persistent or contributing to co-infections, result in significant losses for cattle producers. Although, BVDV can be identified readily by real-time PCR and ELISA, detection and quantification of viral infection at the single cell level is extremely difficult. Detection at the single lymphoid cell level is important due to the immunomodulation that accompanies BVDV infection. A novel PrimeFlow RNA assay using in-situ detection of BVDV was evaluated. The model used to develop this technique included three BL-3 cell lines with different infection statuses, one not infected with BVDV, one infected with BVDV and one dual infected with BVDV and bovine leukosis virus. Using RNA probes specific for the BVDV-2a Npro-Erns coding region, BVDV RNA was detected from both contaminated BL-3 cell lines by flow cytometry and fluorescent microscopy. This is the first report on in-situ detection of BVDV at the single-cell level.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
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Linfócitos
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Vírus da Diarreia Viral Bovina
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Análise de Célula Única
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Citometria de Fluxo
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Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2017
Tipo de documento:
Article