Detection of chronic wasting disease prion seeding activity in deer and elk feces by real-time quaking-induced conversion.
J Gen Virol
; 98(7): 1953-1962, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28703697
ABSTRACT
Chronic wasting disease (CWD) is an emergent prion disease affecting cervid species in North America, Canada, South Korea, and recently, Norway. Detection of CWD has been advanced by techniques that rely on amplification of low levels of prion amyloid to a detectable level. However, the increased sensitivity of amplification assays is often compromised by inhibitors and/or activators in complex biologic samples including body fluids, excreta, or the environment. Here, we adapt real-time quaking-induced conversion conditions to specifically detect CWD prions in fecal samples from both experimentally infected deer and naturally infected elk and estimate environmental contamination. The results have application to detection, surveillance and management of CWD, and potentially to other protein-misfolding diseases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Príons
/
Doença de Emaciação Crônica
/
Fezes
/
Proteínas Priônicas
/
Amiloide
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos