Assay of pyroglutamyl aminopeptidase by high-performance liquid chromatography and its use in peptide sequencing.
Peptides
; 6 Suppl 3: 103-5, 1985.
Article
em En
| MEDLINE
| ID: mdl-2870471
ABSTRACT
Assay of pyroglutamyl aminopeptidase by HPLC methodology allows determination of the kinetic parameters of the enzyme for a variety of natural peptide substrates. Moreover, by this method it is possible both to evaluate the extent of the enzymatic reaction by determining the amount of pyrrolidone carboxylic acid released and to obtain the unblocked peptide for structural and functional characterization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piroglutamil-Peptidase I
/
Aminopeptidases
Limite:
Animals
Idioma:
En
Revista:
Peptides
Ano de publicação:
1985
Tipo de documento:
Article