[Human rhinovirus infection promotes mast cell apoptosis and increases the release of inflammatory cytokines].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 33(7): 886-889, 2017 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-28712394
ABSTRACT
Objective To investigate the regulation of innate immune response of HMC-1 mast cells to human rhinovirus (HRV) infection. Methods Virus replication in HMC-1 cells was quantified by real-time quantitative PCR (qRT-PCR). Cell viability and apoptosis were assessed by flow cytometry. The levels of tumor necrosis factor α (TNF-α), interferon α (IFN-α), interleukin 6 (IL-6) and IL-8 produced by HMC-1 cells were measured using ELISA. Results After HRV infected HMC-1 cells, the copy numbers of virus RNA and apoptotic cells increased obviously over time, what's more, the release of TNF-α, IFN-α, IL-6 and IL-8 from HMC-1 cells ascended significantly compared with control groups. Conclusion HRV infection can promote mast cell apoptosis and enhance the release of inflammatory cytokines from mast cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rhinovirus
/
Citocinas
/
Apoptose
/
Mastócitos
Limite:
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China