Roles of electro-acupuncture in glucose metabolism as assessed by 18F-FDG/PET imaging and AMPKα phosphorylation in rats with ischemic stroke.
Int J Mol Med
; 40(3): 875-882, 2017 Sep.
Article
em En
| MEDLINE
| ID: mdl-28713979
ABSTRACT
Targeted energy metabolism balance contributes to neural survival during ischemic stroke. Herein, we tested the hypothesis that electroacupuncture (EA) can enhance cerebral glucose metabolism assessed by 18Ffluorodeoxyglucose/positron emission tomography (18FFDG/PET) imaging to prevent propagation of tissue damage and improve neurological outcome in rats subjected to ischemia and reperfusion injury. Rats underwent middle cerebral artery occlusion (MCAO) and received EA treatment at the LI11 and ST36 acupoints or nonacupoint treatment once a day for 7 days. After EA treatment, a significant reduction in the infarct volume was determined by T2weighted imaging, accompanied by the functional recovery in CatWalk and Rota-rod performance. Moreover, EA promoted higher glucose metabolism in the caudate putamen (CPu), motor cortex (MCTX), somatosensory cortex (SCTX) regions as assessed by animal 18FFDG/PET imaging, suggesting that threebrain regional neural activity was enhanced by EA. In addition, the AMPactivated protein kinase α (AMPKα) in the CPu, MCTX and SCTX regions was phosphorylated at threonine 172 (Thr172) after ischemic injury; however, phosphorylation of AMPK was further increased by EA. These results indicate that EA could promote AMPKα phosphorylation of the CPu, MCTX and SCTX regions to enhance neural activity and motor functional recovery after ischemic stroke.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Eletroacupuntura
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Isquemia Encefálica
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Glucose-6-Fosfato
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Acidente Vascular Cerebral
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Tomografia por Emissão de Pósitrons
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Proteínas Quinases Ativadas por AMP
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Glucose
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2017
Tipo de documento:
Article