Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein.

Pisal, Rishikaysh V; Hrebikova, Hana; Chvatalova, Jana; Soukup, Tomas; Stanislav, Filip; Mokry, Jaroslav

Pisal, Rishikaysh V; Hrebikova, Hana; Chvatalova, Jana; Soukup, Tomas; Stanislav, Filip; Mokry, Jaroslav.

Afiliação

Pisal RV; Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
Hrebikova H; Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
Chvatalova J; Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
Soukup T; Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
Stanislav F; Department of Oncology and Radiotherapy, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
Mokry J; Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub ; 161(4): 354-359, 2017 Dec.

Article em En | MEDLINE | ID: mdl-28840901

Assuntos

Clonagem Molecular/métodos; Proteínas Luminescentes/genética; Células HeLa; Humanos; Íntrons; Proteínas Luminescentes/metabolismo; Fases de Leitura Aberta; Transfecção; Proteína Vermelha Fluorescente

Palavras-chave

Cyclic ligation assembly (CLA); DsRed2; intron cloned DsRed2

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clonagem Molecular / Proteínas Luminescentes Limite: Humans Idioma: En Revista: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub Assunto da revista: MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: República Tcheca

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