MitoNeoD: A Mitochondria-Targeted Superoxide Probe.
Cell Chem Biol
; 24(10): 1285-1298.e12, 2017 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-28890317
ABSTRACT
Mitochondrial superoxide (O2â
-) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O2â
-, but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed a dual-purpose mitochondrial O2â
- probe, MitoNeoD, which can assess O2â
- changes in vivo by mass spectrometry and in vitro by fluorescence. MitoNeoD comprises a O2â
--sensitive reduced phenanthridinium moiety modified to prevent DNA intercalation, as well as a carbon-deuterium bond to enhance its selectivity for O2â
- over non-specific oxidation, and a triphenylphosphonium lipophilic cation moiety leading to the rapid accumulation within mitochondria. We demonstrated that MitoNeoD was a versatile and robust probe to assess changes in mitochondrial O2â
- from isolated mitochondria to animal models, thus offering a way to examine the many roles of mitochondrial O2â
- production in health and disease.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
Superóxidos
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Revista:
Cell Chem Biol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido