A flow cytometry assay to quantify intercellular exchange of membrane components.
Chem Sci
; 8(8): 5585-5590, 2017 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-28970937
ABSTRACT
Membrane-compound exchange is vital for cell-to-cell communication, yet quantification of this process is difficult. Here we present a method using flow cytometry in combination with bioorthogonal and fluorescent labelling techniques to quantify the amount of exchange of cholesterol and sialylated compounds between cells. We demonstrate that direct cell-cell contact is the likely mechanism of sterol-exchange and show that by manipulating the contact time between cells using complementary coiled-coil peptides results in an enhanced exchange rate of membrane components between cells.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Chem Sci
Ano de publicação:
2017
Tipo de documento:
Article