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Establishment of mouse expanded potential stem cells.
Yang, Jian; Ryan, David J; Wang, Wei; Tsang, Jason Cheuk-Ho; Lan, Guocheng; Masaki, Hideki; Gao, Xuefei; Antunes, Liliana; Yu, Yong; Zhu, Zhexin; Wang, Juexuan; Kolodziejczyk, Aleksandra A; Campos, Lia S; Wang, Cui; Yang, Fengtang; Zhong, Zhen; Fu, Beiyuan; Eckersley-Maslin, Melanie A; Woods, Michael; Tanaka, Yosuke; Chen, Xi; Wilkinson, Adam C; Bussell, James; White, Jacqui; Ramirez-Solis, Ramiro; Reik, Wolf; Göttgens, Berthold; Teichmann, Sarah A; Tam, Patrick P L; Nakauchi, Hiromitsu; Zou, Xiangang; Lu, Liming; Liu, Pentao.
Afiliação
  • Yang J; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Ryan DJ; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Wang W; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Tsang JC; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Lan G; Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge CB2 0RE, UK.
  • Masaki H; Division of Stem Cell Therapy, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
  • Gao X; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Antunes L; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Yu Y; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Zhu Z; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Wang J; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Kolodziejczyk AA; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Campos LS; European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge CB10 1SD, UK.
  • Wang C; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Yang F; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Zhong Z; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Fu B; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
  • Eckersley-Maslin MA; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Woods M; Epigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UK.
  • Tanaka Y; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Chen X; Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.
  • Wilkinson AC; Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 0XY, UK.
  • Bussell J; Division of Cellular Therapy, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
  • White J; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Ramirez-Solis R; Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.
  • Reik W; Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 0XY, UK.
  • Göttgens B; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Teichmann SA; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Tam PPL; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Nakauchi H; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.
  • Zou X; Epigenetics Programme, The Babraham Institute, Cambridge CB22 3AT, UK.
  • Lu L; Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.
  • Liu P; Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 0XY, UK.
Nature ; 550(7676): 393-397, 2017 10 19.
Article em En | MEDLINE | ID: mdl-29019987
Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Blastômeros / Células-Tronco Embrionárias Murinas Limite: Animals / Pregnancy Idioma: En Revista: Nature Ano de publicação: 2017 Tipo de documento: Article País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Blastômeros / Células-Tronco Embrionárias Murinas Limite: Animals / Pregnancy Idioma: En Revista: Nature Ano de publicação: 2017 Tipo de documento: Article País de publicação: Reino Unido