Baicalin alleviates H2O2induced injury of H9c2 cardiomyocytes through suppression of the Wnt/ßcatenin signaling pathway.
Mol Med Rep
; 16(6): 9251-9255, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-29039516
ABSTRACT
Baicalin is one of the active ingredients extracted from the dry root of Scutellaria baicalensis Georgi, which has been reported to be effective in preventing myocardial ischemia reperfusion injury. However, the mechanisms underlying its cardioprotective activities remain to be elucidated. In the present study, H2O2treated cardiomyocyte H9c2 cell line served as an in vitro model of oxidationdamaged cardiomyocytes to evaluate the effects of baicalin on the cardiac injury, and to investigate the underlying molecular mechanism. The results of the TOPFlash/Renilla reporter gene assay indicated that baicalin significantly suppressed the activation of protooncogene Wnt1 (Wnt)/ßcatenin in 293 cells, in a dose dependent manner. In addition, baicalin significantly inhibited H2O2induced loss of H9c2 cell viability in MTT assay. Furthermore, western blotting analysis demonstrated that baicalin markedly attenuated H2O2induced cell apoptosis, as demonstrated by the downregulation of cleaved caspase3 and the increase in the apoptosis regulator Bcl2 (Bcl2)/apoptosis regulator BAX (Bax) ratio following baicalin treatment in H2O2treated H9c2 cells. Furthermore, baicalin markedly decreased the expression of ßcatenin and downstream Axin2 and myc protooncogene protein in H2O2treated H9c2 cells. Knockdown of ßcatenin expression inhibited H2O2induced cell apoptosis. Finally, LiCl (a ßcatenin stabilizer) induced apoptosis of H9c2 cells by upregulating the expression of ßcatenin, which was significantly neutralized by the treatment with baicalin. Taken together, it is hypothesized that baicalin exerts cardioprotective effects via suppression of the Wnt/ßcatenin signaling pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
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Miócitos Cardíacos
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Via de Sinalização Wnt
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Peróxido de Hidrogênio
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Antioxidantes
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2017
Tipo de documento:
Article