Spotting of external calibration standards on blank dried blood spots as a resource-sparing protocol.
Bioanalysis
; 9(19): 1441-1450, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-29056076
AIM: Dried blood spots (DBS) offer significant ethical and scientific advantages; however preparation of calibration curves often times, off-sets some of these advantages. We have developed a methodology wherein small volumes of external calibration standards can be spiked on to blank DBS cards. RESULTS: A total of 2 µl of stock solution spotted on to blank blood spots yielded concentrations that were comparable to those obtained using conventional DBS method. The stability of six analytes on 10-day-old blank spots was within 80-120%. The new methodology was successfully applied to a hydroxycholorquine mouse pharmacokinetics study. CONCLUSION: Blank DBS samples can be opportunistically prepared from overweight or satellite animals, be stored, and subsequently spiked with standards to prepare calibration standards.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Teste em Amostras de Sangue Seco
/
Recursos em Saúde
Tipo de estudo:
Prognostic_studies
Aspecto:
Ethics
Limite:
Animals
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Índia
País de publicação:
Reino Unido