[Single-domain adapted antibodies against Chlamydia trachomatis, preserving the development of chlamidic infection in vitro]. / Odnodomennye adaptirovannye antitela protiv Chlamydia trachomatis, podavliaiushchie razvitie khlamidiinoi infektsii v usloviiakh in vitro.
Biomed Khim
; 63(5): 461-466, 2017 Oct.
Article
em Ru
| MEDLINE
| ID: mdl-29080882
ABSTRACT
The technology for the generating of single-domain recombinant monoclonal antibodies (nanoantibodies) based on the immunization of a camel, cloning of induced sequences encoding single-domain antigen-recognizing fragments of non-canonical camel antibodies, as well as functional selection of clones of nanoantibodies by the phage display method, was used to obtain new effective tools for more efficient diagnostics of Chlamydia infection and to develop new approaches for effective therapy. Two promising nanoantibodies were obtained. They showed effective binding to extracellular and intracellular forms of C. trachomatis, and also had activity that inhibited the development of chlamydial infection in vitro.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Chlamydia
/
Chlamydia trachomatis
/
Anticorpos de Domínio Único
/
Anticorpos Antibacterianos
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
Ru
Revista:
Biomed Khim
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Federação Russa