Your browser doesn't support javascript.
loading
The use of synthetic peptides for detection of anti-citrullinated protein antibodies in rheumatoid arthritis.
Trier, Nicole Hartwig; Holm, Bettina Eide; Heiden, Julie; Slot, Ole; Locht, Henning; Jensen, Bente; Lindegaard, Hanne; Svendsen, Anders; Nielsen, Christoffer Tandrup; Jacobsen, Søren; Theander, Elke; Houen, Gunnar.
Afiliação
  • Trier NH; Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: nhp@ssi.dk.
  • Holm BE; Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark.
  • Heiden J; Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark.
  • Slot O; Department of Rheumatology, Glostrup Hospital, Nordre Ringvej 57, 2600 Glostrup, Denmark.
  • Locht H; Department of Rheumatology, Frederiksberg Hospital, Nordre Fasanvej 57, 2000 Frederiksberg, Denmark.
  • Jensen B; Department of Rheumatology, Frederiksberg Hospital, Nordre Fasanvej 57, 2000 Frederiksberg, Denmark.
  • Lindegaard H; Department of Rheumatology, Odense University Hospital, Søndre Boulevard 29, 5000 Odense C, Denmark.
  • Svendsen A; Epidemiology, Biostatistics and Bio-demography, Institute of Public Health, Southern University, Campusvej 55, 5230 Odense M, Denmark.
  • Nielsen CT; Copenhagen Lupus and Vasculitis Clinic, Center for Rheumatology and Spine Diseases, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark.
  • Jacobsen S; Copenhagen Lupus and Vasculitis Clinic, Center for Rheumatology and Spine Diseases, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark.
  • Theander E; Department of Rheumatology, Skåne University Hospital Malmø, Lund University, Malmø, Sweden.
  • Houen G; Department of Autoimmunology and Biomarkers, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: gh@ssi.dk.
J Immunol Methods ; 454: 6-14, 2018 03.
Article em En | MEDLINE | ID: mdl-29128424
Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. A characteristic feature of RA is the presence of anti-citrullinated protein antibodies (ACPA). Since ACPAs are highly specific for RA and are often present before the onset of RA symptoms, they have become valuable diagnostic and prognostic. As a result, several assays for detection of ACPAs exist, which vary in sensitivity and specificity. In this study, we analyzed the reactivity of RA sera to selected peptides by solid-phase immunoassays in order to develop an ACPA assay with improved sensitivity and specificity. ACPA levels were determined with respect to sensitivity and specificity in 332 serum samples using the newly developed peptide panel, which was compared to the commercial assays CCPlus (Eurodiagnostica) and CCP3.1 (Inova Diagnostics). A primary panel (peptides 814, 33062 and 33156) was identified, which obtained a sensitivity of 71%, while the complete peptide panel reacted with 79% of RA sera screened. Total specificities of 89% and 80% were obtained for the primary peptide panel and the complete peptide panel. Sensitivities for the commercial assays ranged between 71% and 76% and specificities between 88% and 90%. These findings indicate that the generated peptide panel is optimal for ACPA detection and able to compete with commercial available assays. Collectively, this study may contribute to characterize autoimmunity towards citrullinated proteins and to the development of new and improved diagnostic assays for detection of ACPA and determination of RA.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Artrite Reumatoide / Proteoglicanas / Ensaio de Imunoadsorção Enzimática / Anticorpos Antiproteína Citrulinada Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2018 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Artrite Reumatoide / Proteoglicanas / Ensaio de Imunoadsorção Enzimática / Anticorpos Antiproteína Citrulinada Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2018 Tipo de documento: Article País de publicação: Holanda